Determination and Spectroscopy of Quantum Yields in Bio/Chemiluminescence via Novel Light-Collection-Efficiency Calibration: Reexamination of The Aqueous Luminol Chemiluminescence Standard
نویسندگان
چکیده
We have developed a luminescence-measurement system for liquid bio/chemiluminescence that can obtain quantitative luminescence spectra as the absolute total number of luminescence photons at each wavelength or photon energy and quantum yields. Calibration of light-collection efficiency in the system is performed with a reference double-plate cell. This method is applicable to sample cells of any kind suitable for measurement, which is a great advantage over previous techniques in practical experiments. Using this system, the quantum yield of aqueous luminol chemiluminescence was obtained as 1.23 ± 0.20%, which is in good agreement with previously reported values.
منابع مشابه
Studies on PVP hydrogel-supported luminol chemiluminescence: 2. Luminometer calibration and potential analytical applications.
The use of poly(N-vinyl-2-pyrrolidone) (PVP) hydrogel-supported luminol chemiluminescence (CL) for the automatic determination of hydrogen peroxide and the quantification of the antiradical capacity of Trolox is described. The hydrogel containing luminol and hemin is prepared directly on a 96-well microplate and can be stored for up to 3 months without significant decrease in CL quantum yields....
متن کاملChemiluminescence Determination of Atropine using Luminol-Hemin-H2O2 System
A sensitive and fast chemiluminescence (CL) method has been described for the direct determination of atropine. The method is based on the quenching effect of atropine on the CL signals of the luminol– H2O2 reaction catalyzed by hemin in an alkaline medium. Hemin could strongly enhance the chemiluminescence of the luminol–H2O2 system. The luminophor of the luminol–H2O2–hemin CL system was ident...
متن کاملDirect Chemiluminescence Determination of Oxymorphone Using Potassium Permanganate and Polyphosphoric Acid
Abstract A simple and sensitive chemiluminescence (CL) method was developed for direct quantification of oxymorphone, a µ opioid agonist that is approximately 10 times more potent than morphine. In this method potassium permanganate in polyphosphoric acid was used as CL reagent. Using this method, oxymorphone can be determined over the concentration ranges 13.5-337.8 ng mL-1 and 0.34-6.76...
متن کاملAutomated determination of asulam by enhanced chemiluminescence using luminol/peroxidase system.
A flow injection system with chemiluminescence detection for the determination of asulam, enhancer of the system luminol-H(2)O(2)-horseradish peroxidase, is proposed. The method shows a moderate selectivity against other pesticides usually present in formulations of herbicides and in water. The procedure was applied to the determination of asulam in tap water samples and a recovery study was ca...
متن کاملPhotodegradation and flow-injection determination of dithiocarbamate fungicides in natural water with chemiluminescence detection.
A simple and rapid flow-injection method is reported for the determination of dithiocarbamate fungicides (maneb, nabam and thiram) based on chemiluminescence detection. The method involves the photodegradation of dithiocarbamate fungicides via UV light in an alkaline medium. Photoproducts are then reacted with luminol in the absence of an oxidant. Linear calibration graphs were obtained in the ...
متن کامل